Isolation of all soluble tryptic peptides from the alpha polypeptide chain of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and their amino acid compositions.
نویسنده
چکیده
The adult Japanese monkey (macaca fuscata fuscara) hemoglobin was converted into globin by removal of heme. The globin was separated into ƒ¿ and,ƒÀ • @ polypeptide • @ chains • @by • @ countercurrent • @ distribution • @ with • @the • @ solvent system of trichloroacetic acid containing sec-butanol, propionic acid, and water. • @ The • @ ƒ¿ • @ polypeptide • @ chain • @ was • @ hydrolyzed • @ with • @ trypsin. • @ From • @ the hydrolysate, peptides soluble at the adjustment of pH to 6.4 were isolated by column chromatography on Dowex 1 * 2 with acetate buffer containing organic bases such as pyridine, collidine, lutidine, and picoline, and then purified by descending paper chromatography with the mixture of n-butanol, acetic acid, and water. The amino acid compositions of the peptides thus isolated and purified were analyzed after HCl hydrolysis. The results were discussed in comparison with adult human hemoglobin and rhesus monkey (macaca mulatta) hemoglobin. Concerning the amino acid compositions of the soluble tryptic peptides from • @the • @ ƒ¿ • @ polypeptide • @ chain, • @it • @ is • @ presumed, • @that • @ Japanese • @monkey • @ hemo-globin is different from human hemoglobin in four amino acids of the three peptides, but quite the same with rhesus monkey hemoglobin which belongs to the same genus as the Japanese monkey does.
منابع مشابه
Isolation of All Soluble Tryptic Peptides from the α Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions
متن کامل
Isolation of All Tryptic Peptides from the Aminoethylated β Polypeptide Chain of Adult Hemoglobin from Japanese Monkey (Macaca fuscata fuscata) and their Amino Acid Compositions
Hemoglobin prepared from red blood cells of adult Japanese Monkeys (Macaca fuscata fuscata) was removed heme so that globin was obtained. The • @ globin • @ was • @ separated • @into • @ ƒ¿ • @ and ƒÀ polypeptide • @ chains • @ by • @ countercurrent distribution with the system of sec-butanol containing trichroloacetic acid, propionic • @ acid, • @and • @water. • @ The,ƒÀ • @ polypeptide • @ ch...
متن کاملIsolation of all tryptic peptides from the aminoethylated beta polypeptide chain of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and their amino acid compositions.
متن کامل
The N-terminal structure of adult hemoglobin from Japanese monkey (Macaca fuscata fuscata) and the N-terminal amino acids of all the tryptic peptides from its alpha and beta polypeptide chains.
The N-terminal structure of adult Japanese monkey (macaca fuscata fuscata) hemoglobin and the N-terminal amino acid analyses of the tryptic peptides from this hemoglobin were performed. Hemoglobin, after removal of heme, was dinitrophenylated. The DNP-globin was hydrolyzed with HCl, and from the hydrolysate DNP-derivatives were extracted with ether or ethyl acetate. They were isolated and ident...
متن کاملComparative biochemistry of hemoglobins. 8. Isolation of what is called soluble tryptic peptides from the beta-polypeptide chain of Macaca mulatta monkey hemoglobin and their amino acid compositions.
Title Comparative Biochemistry of Hemoglobins VIII. Isolation of What is Called Soluble Tryptic Peptides from the β-Polypeptide Chain of Macaca mulatta Monkey Hemoglobin and their Amino Acid Compositions Author(s) Takei, Hiroshi Citation Acta Medica Nagasakiensia. 1966, 11(1-2), p.84-96 Issue Date 1966-10-25 URL http://hdl.handle.net/10069/17432 Right NAOSITE: Nagasaki University's Academic Out...
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عنوان ژورنال:
- Acta medica Nagasakiensia
دوره 13 3 شماره
صفحات -
تاریخ انتشار 1969